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SEL Number:
07BD-02-DNRN
Title:
Analysis, DNA/RNA Detection
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Last Updated:
6/23/2022 3:14:03 PM
Previous SEL Number:
07BD-02-DNRN
Description:
DNA/RNA detection analysis (example: PCR, ECL).
DIQCode: [D,I]
ImportantFeatures:
Operating Considerations:
Test results are presumptive under field conditions: confirmatory testing at Lab Response Network (LRN) facility required.
Reagent quality: continuous refrigeration may be required, limited shelf life.
Proper sample preparation critical.
Does not discriminate between living and dead organisms.
PCR is highly reliable in detecting substances, with low false positives.
ICostRating: $$$
MCostRating: $$
Training Requirements:
Core Training:
NFPA
National Fire Protection Association
470 Chapter 10.
Initial Training: Extensive (>2 days)
Sustainment Training: Extensive (>2 days)
Mandatory Standards:
Applicable Standards and References:
ASTM E2458-17 Standard Practices for Bulk Sample Collection and Swab Sample Collection of Visible Powders Suspected of Being Biological Agents and Toxins from Nonporous Surfaces
ASTM E2770-17 Standard Guide for Operational Guidelines for Initial Response to Suspected Biological Agents and Toxins
ISO 18385:2016 Minimizing the risk of human DNA contamination in products used to collect, store and analyze biological material for forensic purposes -- Requirements
NFPA 470, Hazardous Materials/Weapons of Mass Destruction (WMD) Standard for Responders, 2022 Edition
NFPA 475: Recommended Practice for Organizing, Managing, and Sustaining a Hazardous Materials/Weapons of Mass Destruction Response Program, 2022 Edition
MSSL:
Responder Safety: Infectious Diseases
REL: Full Canadian Recommended Equipment List
REL: LOS-1, Multi-Agency Intervention
REL: LOS-2, Scout/Reconnaissance Mission
REL: LOS-3, Suspicious Powder Response